RNA-seq and DNA methylomes of germinating rice and developing coleoptiles (Methyl-Seq). RNA-seq and DNA methylomes of germinating rice and developing coleoptiles (Methyl-Seq)

methylC-seq profiling of seeds, at 0 h and 24 h after imbibition and following 4-d of growth under aerobic and anerobic conditions, as well as following re-oxygenation (3dN1dA) in rice. Overall design: gDNA was extracted from samples collected at each time point in triplicate. The time points were: 0 h (dry seed), 24 h and 4 days under aerobic (A) and anaerobic (N) conditions, as well as 3‐day‐old seedlings grown under anaerobic conditions that were switched to air for 1 day.

本数据集针对水稻样本开展甲基胞嘧啶测序（methylC-seq）分析，样本涵盖吸胀后0小时、24小时的种子，以及在好氧、厌氧条件下生长4天的材料，同时包含经厌氧预培养3天后转移至空气环境复氧1天（标注为3dN1dA）的样本。实验整体设计：从各时间点的样本中提取基因组DNA（gDNA），每组设置3次生物学重复。所涉及的时间点与处理条件如下：0小时（干种子）、吸胀后24小时样本，好氧（标注为A）与厌氧（标注为N）条件下生长4天的样本，以及经厌氧条件培养3天后转移至空气环境中复氧1天的3日龄幼苗样本。