Artificial and natural Zika virus populations to benchmark an amplicon-based sequencing protocol to measure intrahost diversity.. Zika virus

We mixed viruses with at known concentrations to benchmark our amplicon-based sequencing protocol to measure intrahost virus diversity. We then explored its utility by sequencing Zika virus from cell culture, mosquito, primate, and human clinical infections and West Nile virus from naturally infected mosquitoes and birds.

我们将已知浓度的病毒混合，以对我们的基于扩增子的测序（amplicon-based sequencing）方案开展基准测试，用于评估宿主内病毒多样性。随后，我们通过对来自细胞培养、蚊虫、灵长类动物及人类临床感染样本的寨卡病毒（Zika virus），以及源自自然感染蚊虫与鸟类的西尼罗河病毒（West Nile virus）进行测序，探究了该方案的应用潜力。