感觉皮层单细胞投射组：环路组织与多感觉整合通路

感觉皮层对于感觉感知和感觉运动行为至关重要，但目前对感觉皮层神经元单细胞水平的投射模式仍知之甚少。在此，我们重建了小鼠躯体感觉、视觉和听觉皮层中5357个单神经元的全脑投射图谱，确定了95种具有独特投射模式的投射组学定义亚型，这些亚型投射至感觉、运动和联合皮层以及丘脑。投射至运动皮层的神经元表现出亚区偏好、拓扑组织以及同侧偏向和对称的双侧投射。投射至丘脑的神经元表现出局灶性或发散性投射模式、依赖层的共支配以及在丘脑网状核中的模态特异性侧支支配。具有跨模态投射的神经元在目标区域和分支模式上多种多样；不同感觉模态神经元投射至联合皮层的轴突末端表现出重叠和层特异性。感觉皮层还形成了层级网络，位于中间层级的部分神经元同时向更高级和更低级层级的脑区投射。我们的研究共同为理解单神经元分辨率下的感觉处理和多感觉整合提供了一个全面的解剖学框架。

The sensory cortex is critical for sensory perception and sensorimotor behaviors. However, our current understanding of the projection patterns of sensory cortical neurons at single-cell resolution remains limited. Here, we reconstructed whole-brain projection maps of 5,357 single neurons in the somatosensory, visual, and auditory cortices of mice, and identified 95 projectionomics-defined subtypes with unique projection profiles. These subtypes project to the sensory, motor, and association cortices, as well as the thalamus. Neurons projecting to the motor cortex show subregion preferences, topographic organization, ipsilateral bias, and symmetric bilateral projections. Neurons targeting the thalamus exhibit focal or divergent projection patterns, layer-dependent co-innervation, and modality-specific collateral innervation within the thalamic reticular nucleus. Neurons with cross-modal projections display diversity in their target regions and branching architectures; axon terminals of neurons from distinct sensory modalities projecting to the association cortices exhibit overlapping patterns and layer-specific distributions. The sensory cortices also form hierarchical networks, wherein some neurons in intermediate layers project to both higher- and lower-level brain regions simultaneously. Collectively, our study establishes a comprehensive anatomical framework for understanding sensory processing and multisensory integration at single-cell resolution.

搜集汇总

背景与挑战

背景概述

该数据集通过重建5,357个小鼠感觉皮层单神经元的投射，识别了95个投射组定义的亚型，揭示了感觉皮层的环路组织和多感觉整合通路。研究聚焦于单细胞分辨率下的投射模式，包括感觉、运动、联合区域以及丘脑的投射特性，为理解感觉处理和多感觉整合提供了全面的解剖框架。