Effects of depletion of Rho-associated, coiled-coil-containing protein kinase 2 in the kidney medullary tussue of murine model.

Rho and its effector Rho-associated, coiled-coil–containing protein kinase 2 (ROCK2) play crucial roles in a variety of cellular functions, such as cell adhesion and cytoskeletal rearrangement . We here investigated the role of ROCK2 in the kidney medullary tissue using tubular specific ROCK2 knockout mice. To investigate the role of ROCK2 in kidney tubular cells, we established the tubular specific ROCK2 knockout mice. RNA-seq on the kidney medullary tissue.

Rho蛋白（Rho）及其效应分子Rho相关卷曲螺旋包含蛋白激酶2（ROCK2）在多种细胞生命活动中发挥关键作用，涵盖细胞黏附与细胞骨架重排等过程。为探究ROCK2在肾小管细胞中的功能，本研究构建了肾小管特异性ROCK2敲除小鼠模型，并利用该模型探讨了ROCK2在肾髓质组织中的作用，同时对肾髓质组织开展了RNA测序（RNA-seq）实验。