Transcriptome-wide investigation of circRNAs in rice.

To obtain sufficient transcriptome data for Oryza sativa L. ssp japonica (Nipponbare), we deeply and individually sequenced polyA selected and polyA depleted samples with technical and biological duplicates from two tissues of mature leaf and panicle. These samples were sequenced by the Illumina ssRNA-seq approach, yielding a total of 710 million paired-end reads sized 100 bp with orientation accuracy from 92.7% to 98.3% that mapped uniquely to the reference genome (IRGSP v5.0).

为获取粳稻（Oryza sativa L. ssp japonica）品种日本晴（Nipponbare）的充足转录组数据，我们针对成熟叶片与穗部两种组织的聚腺苷酸化RNA富集（polyA selected）与聚腺苷酸化RNA去除（polyA depleted）样本开展深度独立测序，所有样本均设置了技术重复与生物学重复。本研究采用Illumina单链RNA测序（ssRNA-seq）方法对上述样本进行测序，最终共获得7.1亿条长度为100 bp的双端读段，其中唯一比对至参考基因组（IRGSP v5.0）的读段定向准确率介于92.7%至98.3%之间。