RNA-Seq data of terminal differentiated cells in Dictyostelium discoideum

The intent of the experiment was to gather transcriptomic data from terminally differentiated cell types --spore, stalk, and cup cells-- in a simple multicellular model system, Dictyostelium discoideum, in order to characterize the cell-type specific gene expression patterns. Specifically, we dissociated and collected each cell type from the fruiting bodies of D. discoideum at 24 hours of development. We also collected exponentially growing vegetative cells of D. discoideum.

本实验旨在从简单多细胞模型系统盘基网柄菌（Dictyostelium discoideum）的终末分化细胞类型——孢子细胞、柄细胞与杯状细胞——中获取转录组数据，以解析细胞类型特异性基因表达模式。具体而言，我们在发育至24小时的D. discoideum子实体中解离并收集了各类细胞。此外，我们还收集了处于指数生长期的D. discoideum营养体细胞。