Airway administration of flagellin regulates the inflammatory response to Pseudomonas aeruginosa

Here, we aim to understand the role of P38 in the airway epithelial cells response to flagellin. We report the gene expression profile of pig airway epithelial cells cultured under air-liquid conditions, pre-incubated or not with the P38 inhibitor SB203580 (20 µM, Tocris Bioscience) for 1 h and then stimulated with 100 ng/ml of a mutated flagellin (FliC?174-400) for 2h or 24h. Our data show that flagellin stimulation induce the expression of pro-inflammatory cytokines mainly through the NFkB pathway, with little impact of P38 inhibition in the cells response. Overall design: RNA-seq analysis of airway epithelial cells pre-incubated (SB) or not (DMSO) with the P38 inhibitor SB203580 and stimulated with 100 ng/ml of flagellin for 2 or 24h.

本研究旨在阐明P38在气道上皮细胞应对鞭毛蛋白（flagellin）时所发挥的作用。本数据集收录了气液共培养条件下培养的猪气道上皮细胞的基因表达谱：将细胞预先以20 μM的P38抑制剂SB203580（购自Tocris Bioscience）孵育1小时，或不进行孵育处理，随后用100 ng/ml的突变型鞭毛蛋白（FliCΔ174-400）刺激2小时或24小时。本研究数据显示，鞭毛蛋白刺激主要通过核因子κB（NFκB）通路诱导促炎细胞因子的表达，而P38抑制对该细胞的应答影响微弱。实验整体设计：对预先用P38抑制剂SB203580孵育（SB组）或未孵育（二甲基亚砜（DMSO）对照组）的气道上皮细胞，以100 ng/ml鞭毛蛋白刺激2小时或24小时后进行RNA测序（RNA-seq）分析。