neonatal rat cardiomyocytes LC-MS/MS

NRCMs were treated with ISO (10 μM) in the presence or absence of FGF18 (50 ng/ml) for 48 h. Protein bands at ~60 kDa were excised from the SDS-PAGE gels for LC-MS/MS analysis

将新生大鼠心肌细胞（NRCMs）在添加或不添加50 ng/ml成纤维细胞生长因子18（FGF18）的体系中，用10 μM异丙肾上腺素（ISO）处理48小时。随后从十二烷基硫酸钠-聚丙烯酰胺凝胶电泳（SDS-PAGE）凝胶中切取分子量约60 kDa的蛋白条带，用于液相色谱-串联质谱（LC-MS/MS）分析。