Selection of Mutant Cell Lines Resistant to Infection by Chlamydia trachomatis and Chlamydia pneumoniae

The lytic outcome of natural infection by Chlamydia trachomatis was exploited to select CHO (Chinese hamster ovary) cells, following chemical mutagenesis, that were deficient in their ability to sustain productive chlamydial infection. Four distinct mutant cell phenotypes with defects in either attachment or postattachment mechanisms that are required for infection by C. trachomatis and Chlamydia pneumoniae were characterized.

本研究利用沙眼衣原体（Chlamydia trachomatis）自然感染的裂解结局，对经化学诱变（chemical mutagenesis）的中国仓鼠卵巢（Chinese hamster ovary, CHO）细胞进行筛选，以获得无法维持衣原体生产性感染的细胞株。随后，本研究对4种不同的突变细胞表型展开系统表征：这些表型的缺陷分别涉及沙眼衣原体与肺炎衣原体（Chlamydia pneumoniae）感染所必需的附着机制（attachment mechanism）或附着后机制（postattachment mechanism）。