Temporal expression study of microRNAs in crown tissues of winter wheat grown under natural growth condition. Temporal expression study of microRNAs in crown tissues of winter wheat grown under natural growth condition

MicroRNAs (miRNAs) are single strand small non-coding RNAs that regulate target mRNAs at post-transcription level. Winter wheat (Triticum aestivum L.), is an important crop plant all over the world. Long term cold exposure (vernalization) is necessary for winter wheat transition from vegetative growth to reproductive growth, yet the involvement of miRNAs in these stages remains unknown. Therefore, we performed next generation sequencing of small RNAs profiles in crown tissues at three-leaf stage, winter dormancy stage, spring greenup stage and jointing stage. Overall design: To identify miRNAs involved in winter wheat growth and development in te field, winter wheat cultivar Shimai 22 was grown in the field under natural weather condition. Crown tissues were collected at 4 different growth and development stages: three leaf stage, winter dormancy stage, spring greenup stage and jointing stage, small RNA libraries were constructed, and BGISEQ500 RS (BGI, China) sequencing platform was used for small RNA libraries sequencing.

微小RNA（miRNAs）是一类单链小型非编码RNA，可在转录后水平调控靶mRNA的表达。冬小麦（Triticum aestivum L.）是全球广泛种植的重要粮食作物。长期低温暴露（春化作用，vernalization）是冬小麦从营养生长向生殖生长转变的必需条件，但目前miRNAs在该进程各阶段中的调控作用仍未明确。为此，本研究针对冬小麦冠部组织在三叶期、冬休眠期、春季返青期以及拔节期的小RNA表达谱开展了高通量测序（next generation sequencing）。试验设计：为筛选参与田间条件下冬小麦生长发育过程的miRNAs，本研究以冬小麦品种石麦22（Shimai 22）为试验材料，在田间自然气象条件下种植。分别于三叶期、冬休眠期、春季返青期以及拔节期这4个生长发育阶段采集冠部组织，构建小RNA文库，并采用BGISEQ500 RS（BGI，中国）测序平台完成小RNA文库测序。