Gene expression analysis by mRNAseq in Fusarium graminearum WT and a DNMT mutant

Identifying the role of two DNA methyltransferase enzymes in Fusarium graminearum isolate NRRL29169 by high-throughput mRNA sequencing under 2 environmental conditions. Two strains of F. graminearum (WT and a DNMT mutant, ∆FgDim-2/∆FgRid) under 2 environmental conditions (24h Preferred nutrient (PN) and 24h Non-preferred nutrient (NPN)) in triplicate.

本研究通过高通量mRNA测序（high-throughput mRNA sequencing）技术，鉴定禾谷镰孢（Fusarium graminearum）菌株NRRL29169中两种DNA甲基转移酶（DNA methyltransferase）在两种环境条件下的功能。实验采用两株禾谷镰孢：野生型（WT）与DNA甲基转移酶（DNMT）突变体∆FgDim-2/∆FgRid，设置2种培养条件，即24小时优选营养（Preferred nutrient, PN）与24小时非优选营养（Non-preferred nutrient, NPN），所有实验组均设置3次生物学重复。