Testicular toxicity induced by carbendazim, iprodione, alone or in combination. Rattus norvegicus

Using an ex-vivo testicular culture from rats, we carried out transcriptomic experiments to identify the pathway of toxicity ellicited by the fungicides carbendazim, iprodione alone or in combination. we used commercial Agilent Microarray GE 4x44K Rat (V3) Gene Expression Microarray (G2514F) AMADID : 028282 Overall design: Cultures were performed with and without fungicides. When required, CBZ, IPR or mixture was added beginning from day 2, at 50 nM or 500 nM for CBZ and at 50 nM or 500 nM for IPR; 50 nM each or 500 nM each for the mixture. For microarray experiments, three different pools of seminiferous tubules were exposed to two concentrations of CBZ, IPR or their mixture (50 nM and 500 nM) or to complete medium with vehicle (control cells) for 7, 14, and 21 days. Each condition of exposure to fungicides was compared with control cells at the same time point.

本研究采用大鼠离体睾丸外植体培养体系，开展转录组学实验，旨在明确杀菌剂多菌灵（carbendazim）、异菌脲（iprodione）单独或联合暴露所诱导的毒性通路。本研究使用商业化安捷伦（Agilent）Microarray GE 4x44K 大鼠（V3）基因表达微阵列（G2514F），其AMADID编号为028282。实验总体设计：培养体系设置杀菌剂处理组与空白对照组。如需进行药物处理，则于培养第2日起添加多菌灵（CBZ）、异菌脲（IPR）或二者混合制剂：单独处理时，多菌灵与异菌脲的浓度均设置为50 nM或500 nM；联合处理时，混合制剂中各组分的终浓度为50 nM或500 nM。针对转录组芯片实验，我们将3份独立的生精小管混合样本分别暴露于2种浓度（50 nM与500 nM）的多菌灵、异菌脲或其联合制剂，同时设置含溶剂的完全培养基对照组，处理时长分别为7、14及21天。所有杀菌剂处理组均与对应时间点的对照组进行比对分析。