Retrospective Analysis of the Beijing Family of Mycobacterium tuberculosis in Preserved Lung Tissues

Direct repeat spoligotyping of 85 paraffin-embedded lung biopsies was used to investigated the occurrence around Beijing of the Beijing family of Mycobacterium tuberculosis. Samples ranged in time from 1956 to 1990. Hybridization patterns were found with 49 (58%) samples, and 45 (92%) produced typical Beijing family patterns extending over the 34-year period.

本研究采用间隔区寡核苷酸分型（spoligotyping）技术，对85例石蜡包埋肺活检组织样本进行检测，以探究结核分枝杆菌（Mycobacterium tuberculosis）北京家族株在北京周边地区的流行情况。样本的采集时间跨度为1956年至1990年。研究共在49例（占比58%）样本中获得有效杂交图谱；其中45例（占比92%）呈现出典型的北京家族株分型特征，该结果覆盖了整个34年的样本采集周期。