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These are the supplemental files for TOXSCI manuscript 19-0578 entitled, “Respirometric Screening and Characterization of Mitochondrial Toxicants Within the ToxCast Phase I and II Chemical Libraries”: Supplemental Figure 1 shows the median fold inhibition for basal (A) and maximal (B) respiration phases for nine test chemicals represented as 18 internally-duplicated samples used to measure intra-assay reproducibility (red dashed lines show threshold used to define bioactivity).<br>Supplemental Figure 2 shows the temporal dose-response plots (median % initial respiration ± mad) for all 243 chemicals tested in Tier 2 RSA screening. <br>Supplemental Figure 3 shows the temporal dose-response plots (median % initial respiration ± mad) for all 149 ETCi chemicals tested in the EFA.<br>Supplemental Table 1 lists five QC metrics calculated using DMSO, fenpyroximate, and 2,4-dinitrophenol controls used to evaluate RSA performance.<br>Supplemental Table 2 lists the 1,042 ToxCast chemicals tested in this study with activity calls for each testing tier, proposed mechanism of mitochondrial toxicity for RSA-actives and the inhibited respiratory complex(es) for putative ETC inhibitors. Maximal test concentrations are in µM excepting those values exceeding 200, which are in µg/µL.<br>Supplemental File A delineates how the respirometric screening assay protocol differs from the commonly-used mitochondrial stress assay protocol and provides a rationale for those differences.

这些是TOXSCI手稿19-0578的补充文件，该手稿标题为《ToxCast I期和II期化学库中线粒体毒物的呼吸测定筛选与表征》：补充图1展示了9种受试化学物质在基础（A）和最大（B）呼吸阶段的中位抑制倍数，这些化学物质以18个内部重复样本的形式呈现，用于衡量批内重复性（红色虚线表示用于定义生物活性的阈值）。<br>补充图2展示了在2期RSA筛选中测试的所有243种化学物质的时间剂量反应曲线（中位初始呼吸百分比±绝对中位差（mad））。<br>补充图3展示了在EFA中测试的所有149种ETCi化学物质的时间剂量反应曲线（中位初始呼吸百分比±绝对中位差（mad））。<br>补充表1列出了使用二甲基亚砜（DMSO）、唑螨酯和2,4-二硝基苯酚对照计算的5项质量控制指标，用于评估RSA性能。<br>补充表2列出了本研究中测试的1042种ToxCast化学物质，包括各测试阶段的活性判定、RSA活性物质的线粒体毒性推测机制以及推定ETCi的受抑制呼吸复合物。测试的最大浓度单位为微摩尔/升（µM），但超过200的值单位为微克/微升（µg/µL）。