Representative Illumina sequencing libraries upon T7-polymerase based amplification

This experiment highlights the extreme sequence bias generated by standard PCR amplication of sequencing libraries and decribes an adapted T7-polymerase based amplification method, which results in non-baised, representative libraries for Illumina sequencing Overall design: Adaptation of standard Illumina sequencing protocol to obtain representative sequencing libraries after sample amplification. Comparing different amplification methods with amplification free sequencing.

本实验揭示了测序文库标准PCR扩增所产生的极端序列偏好性，并描述了一种基于T7聚合酶（T7-polymerase）的改良扩增方法，该方法可获得无偏好性、具有代表性的Illumina测序文库。实验整体设计：对标准Illumina测序流程进行改良，以在样本扩增后获得具有代表性的测序文库；同时比较不同扩增方法与无扩增测序的效果。