A comprehensive study on the co-culture interaction between endophytic Fusarium and host Sanghuangporus

Fungal-fungal interactions frequently result in alterations to the metabolomic profiles of the interacting fungi, with potential ramifications in both industrial and agricultural contexts. In this present study, an endophytic strain of Fusarium sp. MF20 was isolated from the fruiting bodies of Sanghuangporus vaninii. An optimized co-culture system was then established to explore the changes in metabolite profiles resulting from the fungal-fungal interaction, employing a comprehensive analysis integrating transcriptomics and metabolomics. This foundational work paves the way for the enhanced industrial exploitation of Sanghuangporus, a medicinally valuable and rare fungi Overall design: Comparative gene expression profiling analysis using data obtained from RNA-seq of twelve samples in two different treatment groups

真菌间的相互作用常会导致互作真菌的代谢组谱发生改变，该效应在工业与农业场景中均具有潜在影响。本研究从瓦尼桑黄（Sanghuangporus vaninii）的子实体中分离得到一株内生镰孢菌（Fusarium sp.）MF20菌株。随后构建了优化的共培养体系，通过整合转录组学（transcriptomics）与代谢组学（metabolomics）的综合分析手段，探究真菌互作所引发的代谢谱变化。本项基础性研究为药用珍稀真菌桑黄属（Sanghuangporus）的工业化开发利用升级提供了可行路径。整体实验设计：针对两组不同处理下的12份样本的RNA-seq数据，开展比较基因表达谱分析。