To decipher an overall structure of a bacterial anti-plasmid defense system using cross-linking mass spectrometry.

The submitted data is from a BS3 crosslinked sample. The anti-plasmid defense system complex constitutes JetA, JetB, JetC, and JetD subunits. In this work, these four subunits were mixed to form a holo-subunit complex which was followed by crosslinking with isotopically/non-isotopically (1:1) mixture of BS3 reagent. The interactin subunit crosslinks were identified and this information was integrated with alphafold2 enabled models to construct an overall picture of a highly dynamic complex assembly

本次提交的数据来自经BS3交联的样本。该抗质粒防御系统复合物由JetA、JetB、JetC及JetD亚基组成。本研究中将这四种亚基混合形成全亚基复合物，随后使用按1:1比例混合的同位素标记与非同位素标记BS3试剂对其进行交联。研究人员对发生相互作用的亚基交联位点进行了鉴定，并将该信息与AlphaFold2生成的模型相结合，进而构建出该高度动态复合物组装体的整体结构图景。