Vitamin B12 partially rescues embryonic cell migration defects in C. elegans ephrin mutants by improving both propionic acid breakdown and one-carbon cycle metabolic pathways

Cell data files containing nuclei position data for all nuclei detected during embryonic development of C. elegans embryos. In this dataset all embryos are homozygous for the vab-1(e2027) mutation. Embryos were maintained on normal growth media (NGM) with OP50 E. coli or supplemented with 64 nM vitamin B12 (ado-cobalamin). Embryos either hatched or arrested as noted. Embryos carried a transgene driving expression of fluorescent histone protein to identify nuclei position using time-lapse confocal imaging. Images were collected every 1.5 minutes and data was analyzed with StarryNite and manually curated to the 350 cell stage. At this stage and prior, each nucleus represents and individual cell, which is named according to Sulston, 1983.

本数据集的细胞数据文件包含秀丽隐杆线虫（C. elegans）胚胎发育过程中检测到的全部细胞核的位置信息。本数据集内所有胚胎均为vab-1(e2027)突变的纯合个体。实验胚胎分别培养于添加OP50大肠杆菌的标准生长培养基（NGM），或添加64 nM维生素B12（腺苷钴胺素，ado-cobalamin）的培养基中。如实验记录所示，部分胚胎顺利孵化，部分则发育停滞。所有胚胎均携带可驱动荧光组蛋白表达的转基因，以便通过延时共聚焦成像技术定位细胞核位置。图像采集间隔为1.5分钟，数据经StarryNite软件分析，并人工校正至350细胞阶段。在此阶段及更早的发育阶段，每个细胞核对应一个独立细胞，其命名遵循Sulston于1983年提出的命名规范。