Genome-wide H3K56ac profiling after Sirt6 inactivation in MuSCs [ChIP-seq]. Genome-wide H3K56ac profiling after Sirt6 inactivation in MuSCs [ChIP-seq]

Sirt6, the NAD+-dependent deacetylase, has been described to deacetylate H3K9, H3K18, and H3K56. However, analysis of the acetylation status revealed that loss of Sirt6 caused a massive increase of histone H3K56ac levels but no detectable change of histone H3K9ac and H3K18ac, indicating that SIRT6 is the dominant deacetylase for H3K56ac in muscle stem cells (MuSCs). Further, we investigate genome-wide H3K56ac profiling in the absence of Sirt6 in MuSCs and mouse embryonic stem cells (mESCs) using high throughput sequencing (ChIP-seq). Overall design: ChIP-seq experiment to determine genome-wide H3K56ac distribution in control (wild type) and Sirt6 knockout MuSCs

Sirt6作为NAD+依赖型去乙酰化酶（NAD+-dependent deacetylase），已被证实可介导组蛋白H3K9、H3K18及H3K56位点的去乙酰化修饰。然而乙酰化状态分析结果显示，Sirt6缺失会导致组蛋白H3K56ac水平显著升高，但未检测到组蛋白H3K9ac与H3K18ac出现可观测的变化，这表明在肌肉干细胞（Muscle Stem Cells, MuSCs）中，SIRT6是调控H3K56ac的主要去乙酰化酶。后续研究中，我们采用染色质免疫共沉淀测序（ChIP-seq）这一高通量测序技术，对肌肉干细胞与小鼠胚胎干细胞（mouse embryonic stem cells, mESCs）中Sirt6缺失后的全基因组H3K56ac分布谱展开了分析。实验整体设计：本研究通过染色质免疫共沉淀测序（ChIP-seq）实验，明确对照组（野生型）与Sirt6基因敲除肌肉干细胞的全基因组H3K56ac分布特征。