Transcriptome profiling of neutrophils in curdlan-injected Wild Type SKG mice and Mif KO (Mif -/-) SKG mice. Transcriptome profiling of neutrophils in curdlan-injected Wild Type SKG mice and Mif KO (Mif -/-) SKG mice

The curdlan-injected SKG mouse is a mouse model of spondyloarthritis. We identified neutrophils are one of the major immune cells produing MIF in the SKG mice. In this study, we performed total RNA sequecing analysis to identify differentially expressed protein-coding genes (DEGs) in neutrophils isolated from wild type SKG mice comapred to neutrophils isolated from Mif -/- SKG mice after curdlan treatment. Overall design: 4 samples analyzed, 2 neutrophil RNA samples from Wild Type SKG mice and 2 neutrophil RNA samples from MIf KO (Mif -/-) SKG mice.

注射凝胶多糖（curdlan）的SKG小鼠是脊柱关节炎的小鼠模型。本研究证实，中性粒细胞是SKG小鼠体内产生巨噬细胞移动抑制因子（MIF）的主要免疫细胞之一。本研究通过总RNA测序分析，鉴定了经凝胶多糖处理后，野生型SKG小鼠分离的中性粒细胞与Mif基因敲除（Mif -/-）SKG小鼠分离的中性粒细胞之间的差异表达蛋白编码基因（DEGs）。实验设计概述：共分析4份样本，其中2份为野生型SKG小鼠中性粒细胞RNA样本，另外2份为Mif基因敲除（MIf KO, Mif -/-）SKG小鼠中性粒细胞RNA样本。