Six Streptomyces Species Crude Extract LC-MS/MS Analysis For Acyl Homoserine Lactones

This study reports the production of multiple Acyl-Homoserine Lactones (AHLs), quorum sensing molecules thought to be exclusive to Gram-negative, in six Streptomyces species, prominent Gram-positive bacteria, challenging the previously established paradigm. These findings suggest the potential existence of a novel regulatory circuit in Streptomyces, organisms possessing significant secondary metabolism and complex life cycle. These findings hold substantial implications for the industrial, clinical, and evolutionary aspects of Streptomyces. The identification of a new potential regulatory circuit opens avenues for future investigations into the physiological roles of AHLs, the characterization of synthesizing enzymes, and the elucidation of molecular structures in Streptomyces and other Gram-positive bacteria.

本研究报道了在6株链霉菌（Streptomyces）物种中合成多种酰基高丝氨酸内酯（Acyl-Homoserine Lactones，AHLs）的现象——这类群体感应分子此前被认为仅特异性存在于革兰氏阴性菌中，该发现挑战了这一已确立的经典范式。
研究结果提示，链霉菌中可能存在一种全新的调控回路；链霉菌作为一类典型的革兰氏阳性菌，具备显著的次级代谢能力与复杂的生命周期。
本研究发现对链霉菌的工业应用、临床研究以及进化生物学研究均具有深远意义。
这一新型调控回路的鉴定，为未来探索AHLs的生理功能、合成酶的表征以及阐明链霉菌与其他革兰氏阳性菌的分子结构开辟了全新的研究方向。