Genetic suppression reveals DNA repair-independent antagonism between BRCA1 and COBRA1 in mammary gland development

Gene expression profiles of sorted mammary epithelial cells from animals at 4, 6, and 8 weeks of age. Each individual sample was RNA obtained from an individual mouse. Our mice are mammary epithelium specific knocknout mouse models for gene Cobra1 and/or Brca1. For Cobra1, the first four exons were floxed. For Brca1, the largest exon, exon 11, was floxed. The mammary epithelium specific deletions were driven by cre recombinase under MMTV promoter (MMTV-cre). Animal genotype abbreviations used: wild-type (WT), meaning one of the following three: Cobra1f/f (Cff), Brca1f/f (Bff), and Brca1f/f;Cobra1f/f (Dff). Brca1 knockout (BKO), meaning MMTV-cre;Brca1f/f. Cobra1 knockout (CKO), meaning MMTV-cre;Cobra1f/f. Brca1 and Cobra1 double knockout (DKO), meaning MMTV-cre;Brca1f/f;Cobra1f/f Total RNA isolated from CD24+ sorted primary mammary epithelial cells from three time points in different genotypes.

本数据集涵盖4、6、8周龄动物的分选乳腺上皮细胞基因表达谱。每份独立样本均取自单只小鼠的总RNA。所用小鼠为针对基因Cobra1和/或Brca1的乳腺上皮特异性敲除小鼠模型：其中Cobra1基因的前4个外显子经flox位点修饰（floxed），Brca1基因的最大外显子——外显子11经flox位点修饰（floxed）。乳腺上皮特异性基因敲除由MMTV启动子调控的Cre重组酶（Cre recombinase，MMTV-cre）驱动。本研究采用的动物基因型缩写定义如下：野生型（wild-type, WT）包含以下三种：Cobra1f/f（简写为Cff）、Brca1f/f（简写为Bff）以及Brca1f/f;Cobra1f/f（简写为Dff）；Brca1单敲除型（BKO）指基因型为MMTV-cre;Brca1f/f的小鼠；Cobra1单敲除型（CKO）指基因型为MMTV-cre;Cobra1f/f的小鼠；Brca1与Cobra1双敲除型（DKO）指基因型为MMTV-cre;Brca1f/f;Cobra1f/f的小鼠。本数据集的总RNA提取自不同基因型小鼠在三个时间点分选获得的CD24+原代乳腺上皮细胞。