20240305 Gray Figure S6 data

FIG S6 Ability of transition metals (Cu, Zn, Ni) to oxidize TNB. Oxidation of 30 µM 2-nitro-5-thiobenzoic acid (TNB) in 10 mM HEPES-KOH (pH 7.4) by addition of 1 µM CuCl2, ZnSO4, or NiSO4 was measured by loss of absorbance at 412 nm (n=3 experimental replicates; error bars of 1 standard deviation). Asterisks indicate TNB concentrations significantly different from that of TNB with no metal added (one-way ANOVA with Dunnett’s multiple comparisons test; **** = P<0.0001).

图S6 过渡金属（Cu、Zn、Ni）氧化TNB的能力。在10 mM HEPES-KOH缓冲液（pH 7.4）体系中，向30 μM的2-硝基-5-硫代苯甲酸（2-nitro-5-thiobenzoic acid, TNB）内分别加入1 μM氯化铜（CuCl₂）、硫酸锌（ZnSO₄）或硫酸镍（NiSO₄）以启动氧化反应，通过监测412 nm波长处的吸光度衰减量化反应进程（实验重复3次，误差棒代表1倍标准差）。图中星号表示：相较于未添加金属的TNB空白对照组，各实验组的TNB浓度存在显著统计学差异（采用单因素方差分析（one-way ANOVA）结合Dunnett多重比较检验；****对应P<0.0001）。