Insect-specific core virome of Aedes aegypti

Using mosquitoes sampled from Manatee County, FL, USA in 2018, we compared baseline ovary viromes of field (G0) and lab (Orlando) A. aegypti via metagenomic RNA sequencing. Multigenerational maintenance of these ISVs was confirmed orthogonally by RT-PCR in G0 and G7 mosquitoes, indicating vertical transmission as the mechanism for ISV sustentation. This study provides fundamental information regarding ISV ecology, persistence, and variation in A. aegypti in nature.

本研究以2018年采自美国佛罗里达州（FL）马纳蒂县的蚊虫为材料，通过宏基因组RNA测序（metagenomic RNA sequencing），对比了野外种群（G0代）与实验室奥兰多株饲养的埃及伊蚊（A. aegypti）的基线卵巢病毒组。通过逆转录聚合酶链式反应（Reverse Transcription Polymerase Chain Reaction，RT-PCR）对G0代和G7代蚊虫进行正交验证，证实这些昆虫特异性病毒（ISVs，Insect-Specific Viruses）可在蚊体内多代维持，表明垂直传播是昆虫特异性病毒持续存续的机制。本研究为自然界中埃及伊蚊体内昆虫特异性病毒的生态学、持续存活特性及种群变异情况提供了基础科学数据。