A dataset of original image for Western Blot in manuscript of Network Pharmacology Prediction and Experiment Validation to Discover the Mechanism of Sophora Japonica Linn against Liver Cancer

In vitro biological experiments showed that isorhamnetin and quercetin, the main active components of Sophora japonica Linn, had significant inhibitory effect on liver cancer cells HepG2. In addition, the expression of AKT and MEK proteins was downregulated, which proved that both isorhamnetin and quercetin promoted apoptosis by activating the PI3K/AKT and MAPK signaling pathways. In summary, our findings clarify the inhibitory effect of the active ingredients of Sophora japonica Linn against HepG2 cells and provide inspiration for its clinical application in the treatment of liver cancer.

体外生物学实验结果显示，国槐（Sophora japonica Linn）的主要活性成分异鼠李素（isorhamnetin）与槲皮素（quercetin）对肝癌细胞HepG2具有显著抑制效应。此外，AKT及MEK蛋白的表达量显著下调，证实异鼠李素与槲皮素均可通过激活PI3K/AKT与MAPK信号通路促进细胞凋亡。综上，本研究阐明了国槐活性成分对HepG2细胞的抑制作用，为其临床应用于肝癌治疗提供了新思路。