Short Consensus Repeat Domain 1 of Decay-Accelerating Factor Is Required for Enterovirus 70 Binding

Enterovirus 70 (EV70), like several other human enteroviruses, can utilize decay-accelerating factor (DAF [CD55]) as an attachment protein. Using chimeric molecules composed of different combinations of the short consensus repeat domains (SCRs) of DAF and membrane cofactor protein (CD46), we show that sequences in SCR1 of DAF are essential for EV70 binding. Of the human enteroviruses that can bind to DAF, only EV70 and coxsackievirus A21 require sequences in SCR1 for this interaction.

与其他多种人类肠道病毒类似，肠道病毒70型（Enterovirus 70, EV70）可利用衰变加速因子（decay-accelerating factor, DAF [CD55]）作为其附着蛋白。本研究通过构建由DAF与膜辅助蛋白（membrane cofactor protein, CD46）的不同短同源重复结构域（short consensus repeat domains, SCRs）组合而成的嵌合分子，证实DAF的SCR1区域序列是EV70结合所必需的。在可结合DAF的人类肠道病毒中，仅EV70与柯萨奇病毒A21（coxsackievirus A21）需要SCR1区域的序列来完成这一结合相互作用。