Lupinus mutabilis cultivar:sk Transcriptome or Gene expression. Lupinus mutabilis cultivar:Sweet

The research was conducted on embryonic axes which were isolated from imbibed seeds (24 h) and next were cultured for 96 h in the dark under in vitro conditions on a liquid mineral Heller medium with the following trophic variants: i) with 60 mM sucrose (+S), ii) without sucrose (-S), iii) with 60 mM sucrose and 35 mM asparagine (+S+Asn), and iv) without sucrose and with 35 mM asparagine (-S+Asn). Transcriptomic libraries prepared on mRNA isolated from cultured in vitro embryonic axes were sequenced applying the next-generation sequencing (NGS) technique.

本研究以吸胀24小时的种子分离得到的胚轴为实验材料，随后将其置于体外黑暗培养条件下的液体矿物Heller培养基中培养96小时，设置4种营养处理组：i) 添加60 mM蔗糖（+S）、ii) 不添加蔗糖（-S）、iii) 同时添加60 mM蔗糖与35 mM天冬酰胺（+S+Asn）、iv) 不添加蔗糖但添加35 mM天冬酰胺（-S+Asn）。从体外培养的胚轴中分离得到的信使RNA（mRNA）构建转录组文库，并采用下一代测序（next-generation sequencing, NGS）技术完成测序。