Additional file 1 of Targeting human epidermal growth factor receptor 2 enhances radiosensitivity and reduces the metastatic potential of Lewis lung carcinoma cells

Additional file 1: Figure S1. LLC cells divided into HER2-knockdown group and vector-control group were seeded in the Matrigel-coated inserts of Boyden chambers, and treated with sham radiation (no RT) or radiation 7.5Gy (RT) and with or without zoledronic acid (ZA, 30 μM). After 24 h the invading cells were fixed, stained, and viewed by microscope (200X). Invading cells were counted. The effect of MMP9 inhibition on reducing invasiveness were presented by the ratio of invaded cell counts in groups with ZA to groups without ZA.

附加文件1：图S1。将LLC细胞分为HER2敲低组（HER2-knockdown）与载体对照组，接种于包被基质胶（Matrigel）的博伊登小室（Boyden chambers）插入件内，分别施以假照射（无放疗）、7.5Gy放疗（RT）处理，并分别添加或不添加唑来膦酸（zoledronic acid, ZA，30 μM）。培养24小时后，对侵袭至小室基底膜另一侧的细胞进行固定、染色，于200倍光学显微镜下观察并计数。以添加唑来膦酸组与未添加唑来膦酸组的侵袭细胞计数比值，呈现基质金属蛋白酶9（MMP9）抑制作用对细胞侵袭能力的消减效果。