CRISPR and biochemical screens Identify MAZ as a co-factor in CTCF-mediated insulation at Hox Clusters [CRISPR_set9]. CRISPR and biochemical screens Identify MAZ as a co-factor in CTCF-mediated insulation at Hox Clusters [CRISPR_set9]

In this experiment, we sought to analyze the genes positively or negatively selected during differentiation of mouse embryonic stem cells (ESCs) into cervical motor neurons (MNs) with particular focus on the genes enriched for CTCF boundary disruption at HoxA cluster Overall design: Examination of the genes positively or negatively selected in embryonic stem cells and cervical motor neurons using CRISPR loss-of-function screens, with particular focus on the genes enriched for CTCF boundary disruption at HoxA cluster

本实验旨在分析小鼠胚胎干细胞（ESCs）向颈段运动神经元（MNs）分化过程中受到正向或负向选择的基因，重点关注HoxA基因簇处发生CTCF结合边界破坏的富集基因。
总体实验设计：本研究采用CRISPR功能缺失筛选技术，对胚胎干细胞与颈段运动神经元中受到正向或负向选择的基因进行检测，重点关注HoxA基因簇处发生CTCF结合边界破坏的富集基因。