Analysis of Hybrid Incompatability between C nigoni and C briggsae by mRNA sequencing

Two introgression strains (ZZY10307 and ZZY10330) of C. briggsae onto the X chromosome of C. nigoni results in male sterility. In order to determine the cause, we sequenced the mRNAs from young adult males from these two strains, and compared to fertile males of the two parent species (AF16 and JU1421). Two wild-type female samples were also included as platform QC. mRNA profiles of young adult male worms from sterile hybrid strains (ZZY10307 and ZZY10330) and normal fertile parental strains (JU1421 and AF16) were generated by deep sequencing, in triplicate, using Illumina MiSeq V2 kit in 2*150 pair-end format.

将两株布氏隐杆线虫（C. briggsae）的渐渗菌株ZZY10307与ZZY10330整合至尼氏隐杆线虫（C. nigoni）的X染色体后，可引发雄性不育表型。为明确该雄性不育的发生机制，我们对这两株不育菌株的年轻成年雄虫的mRNA进行了测序，并与两个亲本物种（AF16与JU1421）的可育雄虫样本开展对比分析。此外，本研究还纳入两株野生型雌性样本，用于测序平台的质量控制（QC）。本研究采用Illumina MiSeq V2试剂盒，以2×150双端测序模式，对来自不育渐渗杂交菌株（ZZY10307、ZZY10330）以及正常可育亲本菌株（JU1421、AF16）的年轻成年雄虫的mRNA表达谱完成了三次生物学重复的深度测序。