Reference map of Viscum album: Mass spectrometry Data

Recent genomic studies revealed that mitochondria from mistletoe species appear to lack a major amount of genes, indicating a possible loss of the NADH ubiquinone oxidoreductase (complex I) which forms an essential part of the mitochondrial Oxidative Phosphorylation System (OXPHOS). This dataset (mascot generic file (mgf) are gel-based proteomic data: Protein spots from a 2D Blue native - SDS Polyacrylamide gel were carbamidomethylated and in gel digested with trypsin, peptides were extracted and measured with an online Easy nano Liquid Chromatography (Thermo Scientific) - micrOTOF Q II mass spectrometer (Bruker Daltonics) setup. Data were searched with MASCOT (www.matrixscience.com) against an in-house Arabidopsis and Viscaceae database (not shown, see Senkler et al., 2018, Current Biology) and used to build a reference map of the mitochondrial protein complex proteome from European mistletoe, Viscum album. The reference map (available at www.gelmap.de/1327) gives biochemical evidence for a lack of mitochondrial complex I as well as a unique protein (super) complex composition of the oxidative phosphorylation system (OXPHOS) in Viscum album.

近期的基因组学研究显示，槲寄生类物种的线粒体似乎缺失了大量基因，提示其可能丢失了构成线粒体氧化磷酸化系统（Oxidative Phosphorylation System, OXPHOS）核心组分的NADH泛醌氧化还原酶（复合物I，NADH ubiquinone oxidoreductase）。本数据集的MASCOT通用文件（mgf）为基于凝胶的蛋白质组学数据：将二维蓝绿天然-SDS聚丙烯酰胺凝胶分离得到的蛋白斑点进行氨基甲酰甲基化修饰，随后用胰蛋白酶进行胶内酶解；提取肽段后，通过在线Easy纳米液相色谱（Thermo Scientific）- micrOTOF Q II质谱仪（布鲁克道尔顿，Bruker Daltonics）联用系统进行检测。数据通过MASCOT（www.matrixscience.com）检索，比对至本实验室构建的拟南芥与槲寄生科（Viscaceae）数据库（未展示，详见Senkler等，2018，《当代生物学》Current Biology），并用于构建欧洲槲寄生（Viscum album）线粒体蛋白复合物蛋白质组的参考图谱。该参考图谱可于www.gelmap.de/1327获取，其为欧洲槲寄生线粒体缺乏复合物I，以及其氧化磷酸化系统（OXPHOS）拥有独特的蛋白（超级）复合物组成提供了生化依据。