MS data for Huang et al. NCB paper

To identify cofactors of IGF2BPs, we ectopically expressed FLAG-IGF2BP2 in HEK293T cells, pulled down the IGF2BP2 complexes using FLAG antibody and conducted mass spectrometry analysis. Immunoprecipitaion with IgG control and the subsequent mass spectrometry analysis were perform in parallel as a negative control. Proteins identified with 4 or more unique peptides each at 99% confidence are listed in the "Processed data.xlsx" file.

为鉴定胰岛素样生长因子2 mRNA结合蛋白（Insulin-like Growth Factor 2 mRNA-binding Proteins, IGF2BPs）的辅因子，我们在HEK293T细胞中异位过表达FLAG标签融合的IGF2BP2（FLAG-IGF2BP2），采用FLAG抗体下拉富集IGF2BP2复合物并开展质谱分析。同步设置IgG对照免疫沉淀实验并进行后续质谱分析作为阴性对照。以99%置信度鉴定获得、且各包含4条及以上独特肽段的蛋白质均已收录于"Processed data.xlsx"文件中。