HLA-I binding affinity of eluted peptides derived from the invariant chain.

*See Figure 2 for amino acid position in the invariant chain protein.
†IC50 is the concentration used to obtain half maximal competition and represents the mean value of two independent experiments.
‡Binding affinity is classified according to the following IC50 cut-off values: high affinity, ≤5 µM; intermediate (int) affinity, 5–15 µM; low affinity, 15–100 µM; no binding, >100 µM [21].

* 恒定链蛋白（invariant chain protein）的氨基酸位点详见图2。
† 半数抑制浓度（IC50）指可获得半数最大竞争效应的物质浓度，其数值为两次独立实验的平均值。
‡ 结合亲和力（binding affinity）按照以下IC50临界值进行分级：高亲和力（≤5 µM）、中等（int）亲和力（5–15 µM）、低亲和力（15–100 µM）、无结合活性（>100 µM）[21]。