Genome assembly of Cryptococcus gattii VGVI strain WM1802 (Farrer et al mBio 2019). Cryptococcus gattii strain:WM1802

Paired Illumina sequences of C. decagattii (VGVI) were assembled and scaffolded using SPAdes v3.1.1 with k-mer lengths of 21, 33, 55, and 77. A summary of statistics for the assembly is provided in Table 2. Reads were aligned to the assembly with BWA v0.7.4-r385 mem, and Pilon v1.12 was further used to improve the assembly. Scaffolds that were less than 1 kb were removed.

针对C. decagattii (VGVI)的双端Illumina测序读段，采用SPAdes v3.1.1软件，以21、33、55和77作为k-mer长度参数进行基因组组装与支架构建。本次组装的统计汇总信息详见表2。随后利用BWA v0.7.4-r385 mem算法将测序读段比对至该组装序列，并通过Pilon v1.12软件进一步优化组装结果。最终移除了长度小于1 kb的基因组支架。