MouseLight Neuron AA0280

A vast neural tracing effort by a team of Janelia scientists has upped the number of fully-traced neurons in the mouse brain by a factor of 10. Researchers can now download and browse the data in three dimensions.
Inside the mouse brain, individual neurons zigzag across hemispheres, embroider branching patterns and, researchers have now discovered, can even spool out spindly fibers up to 45 centimeters long.
Scientists can see and explore these wandering neural traces in 3-D in the most detailed map of mouse brain wiring yet created. The map reconstructs the shape and position of more than 300 of the 70 million neurons in the mouse brain. Previous efforts to trace the path of individual neurons had topped out in the dozens.
The selectively-labeled neurons were mapped in an iterative process with two-photon microscopy. The brain is sliced in 200-micron sections and a few dozen neurons are labeled at a time and imaged. Each brain imaged yields about 50 terabytes of data, each containing mapped neurons which can be browsed via the NeuronBrowser application. Researchers interested in the complete raw data set should contact Janelia to discuss obtaining it via hardware transfer.

珍妮莉亚（Janelia）研究团队开展的大规模神经示踪工作，将小鼠大脑中已完全示踪的神经元数量提升了10倍。如今研究人员可下载并以三维形式浏览该数据集。
在小鼠大脑中，单个神经元会在大脑半球间蜿蜒穿行，构建出繁复交错的分支形态；研究团队此次还发现，神经元甚至可延伸出长达45厘米的纤细纤维。
这是迄今最为精细的小鼠大脑连接组图谱，科学家可在此以三维形式观察并探索这些蜿蜒的神经示踪轨迹。该图谱重建了小鼠大脑7000万神经元中300余个的形态与位置。此前的单个神经元路径示踪研究，最多仅能完成数十个神经元的示踪。
研究团队采用迭代流程结合双光子显微镜（two-photon microscopy）对选择性标记的神经元进行成像测绘：将大脑切成200微米厚的脑片，每次对数十个神经元进行标记并成像。每枚成像的大脑样本可产生约50太字节（terabytes）的数据，其中包含已完成示踪的神经元数据，可通过神经元浏览器（NeuronBrowser）应用程序进行浏览。若有研究人员需要完整的原始数据集，可联系珍妮莉亚（Janelia）洽谈通过硬件传输的方式获取相关数据。