[CCS and Amyloid A of milk from breast quarters treated in drying with autologous platelet-rich plasma, with or without antibiotics]

ABSTRACT This study aimed to evaluate biomarkers of mastitis after autologous platelet rich plasma (PRP) dry cow therapy, associated or not with antibiotic, compared to conventional treatment. Thirty-six mammary quarters were used in three treatments (T): T1 - antibiotic, T2 - PRP and T3 - PRP + ATB. Milk samples were collected before drying (A1), on calving (D0) and at 14, 30 and 60 days postpartum (D14, D30 and D60), for Somatic Cell Count (CCS) and amyloid dosage A (AA). The design was completely randomized with arrangement in subdivided plots (SigmaPlot ®). Data from CCS were transformed in log10. Means were compared using the Tukey or Holm-Sidak tests, at a 95% confidence level (P<0.05). CCS in A1 was elevated in allgroups (P>0.05). On D30 CCS was higher in T2 (P<0.05), matching D60. There was no difference in AA among T on any day of collection (P>0.05). There was difference in collection moments (P<0.05), A1 different from D14 and D30. There was a weak positive correlation with CCS (0.280). Three treatments were similar in maintaining the health of the mammary gland at subsequent lactation. PRP intramammary can be used for dry cow therapy in subclinical mastitis.

ABSTRACT 本研究旨在评估自体富血小板血浆（platelet rich plasma, PRP）干奶疗法联合或不联合抗生素，与常规疗法相比后，乳腺炎的生物标志物变化。本研究共纳入36个乳腺分区，设置三种处理组（T组）：T1组——抗生素组，T2组——PRP组，T3组——PRP+抗生素组。分别于干奶前（A1）、产犊时（D0）、产后14、30、60天（D14、D30、D60）采集乳样，用于体细胞计数（Somatic Cell Count, CCS）及淀粉样蛋白A（amyloid dosage A, AA）检测。本研究采用完全随机设计，裂区排列（SigmaPlot®）。体细胞计数数据经log₁₀对数转换，采用Tukey检验或Holm-Sidak检验进行组间均值比较，置信水平设定为95%（P<0.05）。A1阶段所有组的体细胞计数均升高（P>0.05）；D30时T2组体细胞计数显著高于其余组（P<0.05），且该差异持续至D60。各处理组间淀粉样蛋白A水平在各采样时间点均无显著差异（P>0.05）；不同采样时间点间存在显著差异（P<0.05），其中A1与D14、D30存在显著差异。淀粉样蛋白A与体细胞计数呈弱正相关，相关系数为0.280。三种处理方案在维持后续泌乳期乳腺健康方面效果相当。乳腺内施用PRP可用于亚临床乳腺炎的干奶疗法。