Gene expression in T1alpha null mutant lungs compared to wild type at dpc 18.5 and term

We compared gene expression in T1alpha (-/-) lungs vs wild type at dpc 18.5 when the phenotype is moderate and at term when the phenotype is more severe. Lungs present narrow and irregular alveolar spaces, thicker mesenchyme, reduced number of attenuated type I cells, normal numbers of type II cells and secreted surfactant. At term, but not a dpc 18.5, there is increased proliferation of distal cells. We compared gene expression of T1alpha null mutant lungs to wild type lungs at dpc 18.5. The mutant phenotype at this developmental stage is moderate but the alveolar sacs are narrower than normal. Keywords: other

本研究于两个时间节点对比了T1alpha基因敲除纯合子（T1alpha (-/-)）肺部组织与野生型（wild type）肺部组织的基因表达差异：其一为表型处于中等程度的孕18.5天（days post coitum，简称dpc），其二为表型更为严重的足月时期。该突变肺组织可见狭窄且不规则的肺泡腔、增厚的肺间质，扁平I型肺泡上皮细胞数量减少，而II型肺泡上皮细胞数量及分泌的肺表面活性物质水平均保持正常。仅在足月时期而非孕18.5天时，远端肺细胞的增殖活性出现升高。本研究还在孕18.5天时，对比了T1alpha基因敲除突变肺组织与野生型肺组织的基因表达。关键词：其他。