Transcript profiling of normal and transformed buccal keratinocytes. Homo sapiens

Normal and two transformed buccal keratinocyte lines were cultured under a standardized condition to explore mechanisms of carcinogenesis and tumor marker expression at transcript and protein level. An approach combining three bioinformatic programs allowed coupling of abundant proteins and large-scale transcript data to low-abundance transcriptional regulators. The analysis identified previously proposed, and suggested novel, protein biomarkers, Gene Ontology categories and molecular networks including functionally impaired key regulator genes for buccal/oral carcinoma. Keywords: Cell type comparison Overall design: Analysis of differential expression in two transformed buccal keratinocyte lines (SVpgC2a and SqCC/Y1) relative to normal buccal keratinocytes. Both normal and transformed cells were cultured under a standardized serum-free condition. Two replicates for all cell types were included.

本研究将正常颊角质形成细胞与两株转化型颊角质形成细胞系在标准化培养条件下进行培养，以探究癌变发生机制以及肿瘤标志物在转录本与蛋白质层面的表达情况。本研究采用整合三款生物信息学程序的分析方法，实现了高丰度蛋白质与大规模转录本数据同低丰度转录调控因子的关联分析。本次分析不仅验证了此前已报道的蛋白质生物标志物、基因本体（Gene Ontology）分类及分子网络，还提出了全新的相关标志物与调控网络，其中包含功能受损的颊/口腔癌关键调控基因。关键词：细胞类型比较 整体实验设计：以正常颊角质形成细胞为对照，分析两株转化型颊角质形成细胞系（SVpgC2a与SqCC/Y1）的基因差异表达情况。所有细胞均在标准化无血清培养条件下进行培养，且每种细胞类型均设置两个生物学重复。