Micro-computed tomography data for: Resolving the design principles that control postnatal vascular growth and scaling

After birth, tissues grow until they reach adult size, with each organ exhibiting unique cellular dynamics, growth patterns, and stem or non-stem cell sources. Using multiscale experimental and computational approaches, we found that aortic enlargement follows distinct growth principles, scaling with the vertebral column. Expansion proceeds via two temporally coordinated, spatially stochastic waves of proliferation aligned with blood flow, each with unique cell-cycle kinetics, with the first wave featuring cycles as short as 6 h. Single-cell RNA sequencing revealed increased fatty acid metabolism accompanying cell enlargement. Mathematical modeling and experiments showed that endothelial cell extrusion is essential for maintaining homeostatic aortic size as it adjusts for proliferation excess. Using a genetic model of achondroplasia, we mechanistically demonstrated that the aorta preserves proper scaling by increasing cell extrusion while keeping proliferation rates intact. These findin..., , # Resolving the design principles that control postnatal vascular growth and scaling

Dataset DOI: [10.5061/dryad.kh18932kp](10.5061/dryad.kh18932kp)

Data were collected as described in the associated publication.

Sam Brennan and Stuart R. Stock (Northwestern University) collected the microCT data.

Stuart Stock made the measurements of aortic and vertebral lengths.

Stuart Stock curated the data sets.

**Description of the data and file structure**

microCT scan of mouse spines and aortas filled with contrast agent using a Bruker SkyScan 1272

### Files and variables

1\) microCT slices and metadata for wild type mice

2\) microCT slices and metadata for osx-cre mice. plus and minus denote KO and nonexpressing

This data set consists of 1) microCT slices and 2) their associated meta-data for the Cell Systems paper “Resolving the design principles that control postnatal vascular growth and scaling.” Danielle Pi, Jonas Braun, et al. (in press 2025). The data consist of image sets of th...,

出生后，机体组织持续生长直至达到成年个体体型，各器官均具备独特的细胞动态特征、生长模式以及干细胞或非干细胞来源。我们采用多尺度实验与计算研究手段，发现主动脉扩张遵循独特的生长法则，其尺寸与脊柱长度呈比例匹配。扩张过程通过两个在时间上协调、空间上随机分布的增殖波推进，该增殖波的走向与血流方向一致，二者各自具备独特的细胞周期动力学特征，其中第一波的细胞周期可短至6小时。单细胞RNA测序（single-cell RNA sequencing，scRNA-seq）结果显示，细胞体积增大的同时伴随脂肪酸代谢水平的上调。数学建模与实验均证实，内皮细胞挤出（endothelial cell extrusion）对于维持主动脉的稳态尺寸至关重要，因其可抵消过度增殖带来的影响。我们借助软骨发育不全（achondroplasia）遗传模型，从机制层面证实：主动脉可通过增加细胞挤出量、同时维持增殖速率不变的方式，实现恰当的比例缩放。本研究的发现……
# 解析调控出生后血管生长与比例缩放的设计原则

数据集DOI：[10.5061/dryad.kh18932kp](10.5061/dryad.kh18932kp)

数据采集方式详见相关发表论文。

Sam Brennan与Stuart R. Stock（美国西北大学）完成了显微CT（microCT）数据的采集工作。Stuart Stock负责主动脉与脊柱长度的测量，并完成了数据集的整理工作。

**数据与文件结构说明**
使用布鲁克SkyScan 1272（Bruker SkyScan 1272）扫描仪，对填充了造影剂的小鼠脊柱与主动脉开展显微CT扫描。

### 文件与变量
1. 野生型小鼠的显微CT切片与元数据（metadata）
2. osx-cre小鼠的显微CT切片与元数据，其中"plus"与"minus"分别代表基因敲除（KO）样本与无基因表达样本

本数据集包含1) 显微CT切片，以及2) 其配套的元数据，源自发表于《细胞系统》（Cell Systems）的论文《解析调控出生后血管生长与比例缩放的设计原则》，作者为Danielle Pi、Jonas Braun等，2025年待刊。该数据集包含……相关的图像集