Gene expression profiles in CD44- and CD44+ hepatocellular carcinoma HuH7 cells. Homo sapiens

To investigate the significance of CD44+ hepatocellular carcinoma (HCC) HuH7 cells, gene expression profiles of CD44-positive HuH7, CD44-negative HuH7, and human nomal hepatocyes were analyzed. Results provide the insight into the significance of CD44-positive HCC cells as the liver CSCs. Overall design: CD44-positive and CD44-negative HuH7 cells were sorted by flow cytometry. Total RNA were extracted from CD44-positive HuH7, CD44-negative HuH7 cells, and human normal hepatocytes. Total RNA isolated from each cell labeled using Cy5 and hybridized onto the 3D-Gene human mRNA oligo chip (human 25k, Ver 2.10).

为探究CD44阳性肝细胞癌（hepatocellular carcinoma, HCC）HuH7细胞的关键生物学意义，本研究对CD44阳性HuH7细胞、CD44阴性HuH7细胞以及正常人肝细胞的基因表达谱展开了分析。本研究结果可为阐明CD44阳性肝癌细胞作为肝脏肿瘤干细胞（Cancer Stem Cells, CSCs）的重要意义提供新的理论依据。
整体实验设计：通过流式细胞术分选出CD44阳性与阴性的HuH7细胞；从CD44阳性HuH7细胞、CD44阴性HuH7细胞及正常人肝细胞中提取总RNA；将每份样本分离得到的总RNA经Cy5标记后，杂交至3D-Gene人mRNA寡核苷酸芯片（人25k，版本2.10）上。