Avermectin treatment for Lepeophtheirus salmonis: Impacts on host (Salmo salar) and parasite immunophysiology

This study investigates the transcriptomic responses of salmon lice (L. salmonis) infecting salmon treated with emamectin benzoate (either 1x or 3x), ivermectin, or commercial feed controls Copepodid-staged larvae of L. salmonis were used to infect Atlantic salmon fed 1x or 3x emamectin benzoate, ivermectin, or commercial feed controls. Pre-adult I lice were collected from each group, pooled by treatment group and sex (pools of 6 lice) and stored at -80C for RNA extraction and microarray analyses. A total of 48 pools were used (n = 6 per condition except n = 5 for male controls and n = 7 for male 3xEMB)

本研究针对感染经苯甲酸盐依维菌素（emamectin benzoate，1倍或3倍剂量）、伊维菌素（ivermectin）处理，或喂食商业对照饲料的大西洋鲑的鲑鱼虱（L. salmonis）的转录组响应展开探究。本研究采用桡足幼体期（Copepodid-staged）的L. salmonis感染喂食了1倍或3倍剂量苯甲酸盐依维菌素、伊维菌素或商业对照饲料的大西洋鲑。随后从各组中收集预成虫I期的鲑鱼虱，按处理组与性别进行混样（每6只个体组成一个混样），并置于-80℃低温保存以用于RNA提取与微阵列（microarray）分析。本研究共计使用48个混样，其中除雄性对照组n=5、雄性3倍剂量苯甲酸盐依维菌素组n=7外，其余各组n均为6。