Multi-organ RNAseq Data

Raw and process multi-organ RNAseq datasets for use in a Genomics Data Carpentry workshop. Raw data was collected from the NCBI SRA, corresponding to the study conducted by Ruiz-Orera et al. (2015). Data was processed to create logical intermediate files to aid in keeping students on track.<br>The 'reference' directory contains the reference transcriptome of all RefSeq transcripts for the NCBI human genome.<br>The 'untrimmed_fastq' contains the raw FASTQ data for the human samples from this study, which are significantly subsetted to make them efficient for analysis during a workshop.<br>There is also a hidden '.backup' directory containing quality-trimmed and mapped files for each sample so they are available to aid student learning.

本数据集为面向基因组数据Carpentry教学工坊（Genomics Data Carpentry）打造的多器官RNA测序（RNA-seq）原始与处理数据集。原始数据采集自美国国家生物技术信息中心（National Center for Biotechnology Information, NCBI）的序列读取档案（Sequence Read Archive, SRA），对应Ruiz-Orera等人2015年开展的研究。为协助学员跟上教学节奏，我们对数据进行了处理，生成了逻辑清晰的中间文件。
"reference"目录包含适配NCBI人类基因组的全部参考序列（RefSeq）转录本的参考转录组。
"untrimmed_fastq"目录收录了本研究中人类样本的原始FASTQ测序数据，该数据集已进行大幅子集化精简，以提升工坊教学中的分析效率。
此外还设有一个隐藏的".backup"目录，其中包含各样本的质量修剪与序列比对文件，可用于辅助学员学习。