Signaling pathways activated by PDGF-AA, PDGF-BB, HB-EGF, TCM, or VECM.

(A) Decidualized St-T1b were starved in Opti-MEM and then treated for 5 or 30 min with PDGF-BB, HB-EGF, TCM or PDGF-AA. Levels of phosphorylated (p-) or total ERK1/2, Akt, and p38 were determined by Western blotting. (B) Decidualized hESC were starved in Opti-MEM and then treated for 5 min with PDGF-BB, HB-EGF, TCM, 5 individual VECM preparations, villous explant control medium (VECM-Co), or PDGF-AA. Western blotting was performed as above.

(A) 将蜕膜化St-T1b细胞置于Opti-MEM培养基中进行饥饿处理，随后分别以血小板衍生生长因子BB（PDGF-BB）、肝素结合性表皮生长因子（HB-EGF）、条件培养基（TCM）及血小板衍生生长因子AA（PDGF-AA）处理5分钟或30分钟；采用蛋白质印迹法（Western blotting）检测磷酸化（p-）细胞外信号调节激酶1/2（ERK1/2）与总ERK1/2、蛋白激酶B（Akt）与总Akt、p38丝裂原活化蛋白激酶（p38）与总p38的蛋白水平。(B) 将蜕膜化人子宫内膜基质细胞（hESC）置于Opti-MEM培养基中进行饥饿处理，随后分别以PDGF-BB、HB-EGF、TCM、5份独立制备的VECM制剂、绒毛外植体对照培养基（VECM-Co）及PDGF-AA处理5分钟；按照前述方法开展蛋白质印迹实验。