Hybrid aspen (Populus tremula x tremuloides)1h cytokinin induction and 1h control treatment data.

Aim of the project: Genome wide gene expression analysis for cytokinin (100nM 2ip, 20nM NaPi buffer) fast (1h) response genes from 3 months old Hybrid aspen(Populus tremula x tremuloides)apical part (30 cm from tip; diameter:4-5mm) of stem. Stems of two individuals (trees 15 and 16) was cut into 50-100um thick (free hand)cross sections randomly selected stem discs are set to two pools: cytokinin treatment and control treatment. Samples are collected noon time (11:00-11:30). Cytokinin treatment: stem discs (several hundred) are submerged with 100nM 2ip, 20nM NaPi buffer, with modest shaking for 60 min +/- 2 min (time to collect about 30mg stem discs (several dozens) for RNA sample. Control treatment: identical to cytokinin treatment, only without 100nM 2ip.

本项目的研究目标为：针对3月龄杂种杨（Populus tremula x tremuloides）茎尖部（距顶端30cm，直径4-5mm）的细胞分裂素（100nM 2ip，20nM磷酸钠缓冲液）1小时快速响应基因开展全基因组基因表达分析。选取编号为15和16的两株杨树的茎段，徒手切成厚度为50-100μm的横切片，将随机选取的茎盘分为两组：细胞分裂素处理组与对照组。样本采集于正午时段（11:00-11:30）。细胞分裂素处理组：将数百个茎盘浸没于含100nM 2ip的20nM磷酸钠缓冲液中，温和振荡60分钟±2分钟，随后收集约30mg茎盘（约数十个）用于RNA样本制备。对照组：处理流程与细胞分裂素处理组完全一致，仅不添加100nM 2ip。