Gene Expression Dynamics in Late Embryonic and Early Postnatal Mouse Ovaries. Gene Expression Dynamics in Late Embryonic and Early Postnatal Mouse Ovaries

This analysis represents one of the first unbiased sampling of germ cells in the developing murine ovary over time, at high-resolution, single-cell depth, revealing novel genetic regulatory signatures of murine female germ cells and somatic cells over time. Overall design: Single-cell suspensions of mammalian ovaries ranging from E18.5 to PND5 were created, processed for 10x Genomics and sequencing on an Illumina NextSeq.

本分析为首批针对发育中小鼠卵巢生殖细胞开展的时序高分辨率单细胞无偏采样研究之一，揭示了小鼠雌性生殖细胞与体细胞随发育进程变化的新型基因调控特征。实验设计概述：制备了胚胎期18.5天至出生后第5天的哺乳动物卵巢单细胞悬液，经10x Genomics平台处理后，在Illumina NextSeq测序仪上完成测序。