Genome sequence resources from three isolates of the apple canker pathogen Neonectria ditissima infecting forest trees

Neonectria ditissima is a generalist ascomycete plant pathogen causing canker diseases on a variety of hardwood tree species and can cross-infect many of them. The fungus enters the plants through wounds throughout the year. N. ditissima is considered a major threat to apple production responsible for the fruit tree canker disease which damages trees and causes rotting of fruits in storage. Nearby forests and shelter belts can serve as source of inoculum for well-managed apple orchards. Thus, knowledge about the N. ditissima isolates infecting different host species is essential for designing integrated pest management strategies. Here, we describe the genomes of three N. ditissima isolates, Nd_iso34, Nd_iso35, and Nd_iso36, infecting European beech, American tulip tree, and American beech, respectively. We obtained genome assemblies of ca. 45 megabases for all isolates, covering 94% of the N. ditissima reference annotation, and 97% of the universal single-copy orthologs (BUSCOs). We conclude that these genome assemblies are a highly relevant resource considering the scarcity of genomic data available for N. ditissima.

Neonectria ditissima是一种泛寄主型子囊菌（ascomycete）植物病原菌，可引发多种阔叶树种的溃疡病，并能在诸多宿主间交叉侵染。该真菌可全年通过伤口侵入植物体内。N. ditissima被视为苹果产业的主要威胁之一，其引发的果树溃疡病不仅会造成树木损伤，还会导致贮藏期果实腐烂。周边森林及防护林带可作为管理良好的苹果园的病原菌接种体（inoculum）来源。因此，解析侵染不同宿主的N. ditissima分离株的相关信息，对于制定综合有害生物管理策略至关重要。本研究报道了3株N. ditissima分离株的基因组序列：Nd_iso34、Nd_iso35和Nd_iso36，分别侵染欧洲山毛榉、北美鹅掌楸及美国山毛榉。所有分离株的基因组组装（genome assembly）大小约为45兆碱基（Mb），覆盖了94%的N. ditissima参考注释序列，以及97%的通用单拷贝直系同源基因（BUSCO）。鉴于目前已公开的N. ditissima基因组数据较为匮乏，本研究获得的基因组组装资源具有极高的参考价值。