Molecular characterization of murine IFN producing killer dendritic cells. Mus musculus

IKDCs have been newly characterized in mouse and shared functional and phenotypic properties of NK cells and dendritic cells. To formaly distinguish them from NK cells, we have performed a gene expression profile analysis of splenic and LN cell sorted IKDC and compared them with CDC and NK.We are looking for gene or group of genes specifically expressed by IKDCs and which would allow us to specifically isolate and characterize them. Keywords: Freshly isolated cells Overall design: IKDC, NK and CDC were cell-sorted from spleen and lymph node (LN) of naïve BALB/c mice (6-8 weeks).

产干扰素杀伤树突状细胞（IKDCs）是近期在小鼠中被鉴定的一类细胞，兼具自然杀伤细胞（NK cells）与树突状细胞（dendritic cells）的功能及表型特征。为正式将其与自然杀伤细胞区分开来，本研究对脾脏及淋巴结（lymph node, LN）中分选得到的产干扰素杀伤树突状细胞开展了基因表达谱分析，并与常规树突状细胞（conventional dendritic cells, CDC）及自然杀伤细胞进行对比。本研究旨在筛选该细胞特异性表达的基因或基因集，以实现对其特异性分离与鉴定。关键词：新鲜分离的细胞；实验设计概述：从6-8周龄未免疫BALB/c小鼠的脾脏及淋巴结中分选得到产干扰素杀伤树突状细胞、自然杀伤细胞及常规树突状细胞。