MOESM9 of Wild-type Cu/Zn-superoxide dismutase is misfolded in cerebrospinal fluid of sporadic amyotrophic lateral sclerosis

Additional file 9. A spreadsheet with ELISA data was available in a Microsoft Excel file. In a “Standard curve” sheet, absorbance values at 490 nm for preparation of standard curves (i.e. Additional file 3: Figure S2) were summarized. Also, absorbance values at 490 nm in the sandwich ELISA on CSF were available in a “Absorbance” sheet. As described in the Methods, furthermore, absorbance values at 490 nm measured in the ELISA were substituted into equations fitted to standard curves (Additional file 3: Figure S2), and apparent amounts of SOD1 were summarized in an “Apparent amount” sheet (i.e. Figures 3 and 6). In all sheets, averages together with standard deviations of the observed data were shown.

附加文件9：本文件为包含酶联免疫吸附试验（Enzyme-Linked Immunosorbent Assay, ELISA）数据的Microsoft Excel电子表格。其"标准曲线"工作表汇总了用于绘制标准曲线的490nm波长处吸光度值（对应附加文件3：图S2）。"吸光度"工作表收录了脑脊液（Cerebrospinal Fluid, CSF）样本夹心ELISA（sandwich ELISA）检测所得的490nm波长处吸光度值。如方法章节所述，本研究将ELISA检测得到的490nm吸光度值代入标准曲线拟合方程（对应附加文件3：图S2），并将计算得到的超氧化物歧化酶1（Superoxide Dismutase 1, SOD1）表观含量汇总至"表观含量"工作表中（对应图3与图6）。所有工作表均展示了观测数据的平均值与标准差。