H3K4me3 (ab8580 lot1).Kc

modENCODE_submission_3761 This submission comes from a modENCODE project of Gary Karpen. For full list of modENCODE projects, see http://www.genome.gov/26524648 Project Goal: We aim to determine the locations of the major histone modifications across the Drosophila melanogaster genome. The modifications under study are involved in basic chromosomal functions such as DNA replication, gene expression, gene silencing, and inheritance. We will perform Chromatin ImmunoPrecipitation (ChIP) using genomic tiling arrays. We will initially assay localizations using chromatin from three cell lines and two embryonic stages, and will then extend the analysis of a subset of proteins to four additional animal tissues/stages. For data usage terms and conditions, please refer to http://www.genome.gov/27528022 and http://www.genome.gov/Pages/Research/ENCODE/ENCODEDataReleasePolicyFinal2008.pdf EXPERIMENT TYPE: CHIP-chip. BIOLOGICAL SOURCE: Cell Line: S2-DRSC; Tissue: embryo-derived cell-line; Developmental Stage: late embryonic stage; Sex: Male; NUMBER OF REPLICATES: 4; EXPERIMENTAL FACTORS: Cell Line S2-DRSC; Antibody H3K4me3 (ab8580 lot1) (target is H3K4me3)

modENCODE_submission_3761 本提交源自Gary Karpen的modENCODE项目。如需查阅modENCODE项目的完整列表，请访问http://www.genome.gov/26524648。项目目标：本研究旨在定位黑腹果蝇（Drosophila melanogaster）全基因组范围内的主要组蛋白修饰位点。所研究的修饰参与DNA复制、基因表达、基因沉默及遗传等基础染色体功能。本研究将采用基因组平铺阵列开展染色质免疫沉淀（Chromatin ImmunoPrecipitation，ChIP）实验。研究初期将使用三种细胞系与两个胚胎阶段的染色质进行位点定位分析，后续将针对部分蛋白质，将分析范围拓展至另外四种动物组织/发育阶段。关于数据使用的条款与条件，请参阅http://www.genome.gov/27528022 及 http://www.genome.gov/Pages/Research/ENCODE/ENCODEDataReleasePolicyFinal2008.pdf。实验类型：CHIP-chip。生物样本来源：细胞系：S2-DRSC；组织：胚胎来源细胞系；发育阶段：晚期胚胎阶段；性别：雄性；生物学重复次数：4次；实验因素：细胞系S2-DRSC；抗体：H3K4me3（ab8580 批次1），靶标为H3K4me3