Evaluation of p-Naphtholbenzein-β-d-Galactoside as a Substrate for Bacterial β-Galactosidase

We describe the synthesis of a new substrate for the detection of β-galactosidase and evaluate its performance in comparison with 5-bromo-4-chloro-3-indolyl-β-d-galactopyranoside (X-Gal) and cyclohexenoesculetinβ-d-galactoside (CHE-Gal). Of 206 Enterobacteriaceae strains able to hydrolyze X-Gal, 194 (94.2%) hydrolyzed CHE-Gal and 192 (93.2%) hydrolyzed p-naphtholbenzein-β-d-galactoside (PNB-Gal). We conclude that PNB-Gal is an effective substrate for the detection of β-galactosidase.

本研究报道了一种用于检测β-半乳糖苷酶（β-galactosidase）的新型底物的合成，并将其性能与5-溴-4-氯-3-吲哚基-β-D-半乳糖苷（5-bromo-4-chloro-3-indolyl-β-d-galactopyranoside，简称X-Gal）以及环己烯没食子酸-β-D-半乳糖苷（cyclohexenoesculetinβ-d-galactoside，简称CHE-Gal）进行了对比评估。在206株可水解X-Gal的肠杆菌科（Enterobacteriaceae）菌株中，194株（94.2%）可水解CHE-Gal，192株（93.2%）可水解对萘酚苯-β-D-半乳糖苷（p-naphtholbenzein-β-d-galactoside，简称PNB-Gal）。本研究得出结论：PNB-Gal是一种用于检测β-半乳糖苷酶的有效底物。